Recombinant Mouse GP6 Protein (Met1-Lys265), C-6×His-tagged
Product Description
Cat
IMP-10143
Official Symbol
GP6
Product Overview
Recombinant Mouse GPVI protein (Met1-Lys265) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Gly24
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 27.8 kDa (monomer)
SDS-PAGE: 45-60 kDa, reducing conditions
Protein length
Met1-Lys265
Bio-activity
Measured by its binding ability in a functional ELISA.
When cross-linked with 10 μg/mL of Mouse Anti-polyHistidine Monoclonal Antibody, Recombinant Mouse GPVI binds to Collagen I (coated at 10 μg/mL, 100 μL/well) with an apparent KD <90 nM.
Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Notes
Noncovalently-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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