Recombinant Mouse GPC1 Protein (Asp24-Ser529), C-6×His-tagged

Product Description

Cat

IMP-10145

Official Symbol

GPC1

Product Overview

Recombinant Mouse Glypican 1 protein (Asp24-Ser529) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Asp24

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 56.4 kDa
SDS-PAGE: 57-62 kDa, reducing conditions

Protein length

Asp24-Ser529

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 1 at 3 μg/mL (100 μL/well) will bind rhFGF-basic with an apparent KD < 1 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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