Recombinant Mouse GUCY2C Protein (Val20-Gln430), C-6×His-tagged

Product Description

Cat

IMP-10154

Official Symbol

GUCY2C

Product Overview

Recombinant Mouse Guanylyl Cyclase C/GUCY2C protein (Val20-Gln430) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive).

Expression System

CHO

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Val20

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Molecular Mass

Predicted Molecular Mass: 48 kDa
SDS-PAGE: 85-100 kDa, under reducing conditions.

Protein length

Val20-Gln430

Bio-activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in PBS.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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