Recombinant Mouse ICAM1 Protein (Gln28-Asn485), C-6×His-tagged

Product Description

Cat

IMP-10605

Official Symbol

Icam1

Product Overview

Recombinant Mouse ICAM-1/CD54 protein (Gln28-Asn485) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Gln28

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 51 kDa
SDS-PAGE: 85-105 kDa, under reducing conditions

Protein length

Gln28-Asn485

Bio-activity

Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.075-0.6 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.075-0.6 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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