Recombinant Mouse ICAM1 Protein (Gln28-Asn485), C-6×His-tagged

Product Description

Cat

IMP-10605

Official Symbol

Icam1

Product Overview

Recombinant Mouse ICAM-1/CD54 protein (Gln28-Asn485) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Gln28

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 51 kDa
SDS-PAGE: 85-105 kDa, under reducing conditions

Protein length

Gln28-Asn485

Bio-activity

Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.075-0.6 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.075-0.6 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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