Recombinant Mouse ICOS Protein (Glu21-Leu142), C-hFc-tagged

Product Description

Cat

IMP-10410

Official Symbol

Icos

Product Overview

Recombinant Mouse ICOS protein (Glu21-Leu142) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Sf 21 cells.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

Sf 21 cells

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 44 kDa (monomer)
SDS-PAGE: 47 kDa, reducing conditions

Protein length

Glu21-Leu142

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rmICOS/Fc Chimera at 1 μg/mL (100 μL/well) can bind biotinylated rhB7-H2/Fc Chimera with a linear range of 0.156-10 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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