Recombinant Mouse ICOS Protein (Glu21-Leu142), C-hFc-tagged

Product Description

Cat

IMP-10410

Official Symbol

Icos

Product Overview

Recombinant Mouse ICOS protein (Glu21-Leu142) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Sf 21 cells.

Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Expression System

Sf 21 cells

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Glu21

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 44 kDa (monomer)
SDS-PAGE: 47 kDa, reducing conditions

Protein length

Glu21-Leu142

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rmICOS/Fc Chimera at 1 μg/mL (100 μL/well) can bind biotinylated rhB7-H2/Fc Chimera with a linear range of 0.156-10 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry