Recombinant Mouse ICOSLG Protein (Glu47-Lys279), C-6×His-tagged

Product Description

Cat

IMP-10416

Official Symbol

Icosl

Product Overview

Recombinant Mouse B7-H2 protein (Glu47-Lys279) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Glu47

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 27 kDa
SDS-PAGE: 50-70 kDa, reducing conditions

Protein length

Glu47-Lys279

Bio-activity

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 200 μg/mL in PBS.

Bioactivity-ELISA 1

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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