Recombinant Mouse ICOSLG Protein (Glu47-Lys279), C-hFc-tagged

Product Description

Cat

IMP-10417

Official Symbol

Icosl

Product Overview

Recombinant Mouse B7-H2 protein (Glu47-Lys279) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Glu47

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 53 kDa (monomer)
SDS-PAGE: 80-90 kDa, reducing conditions

Protein length

Glu47-Lys279

Bio-activity

Measured in a competitive binding assay. When rmICOS/Fc Chimera is immobilized at 1 μg/mL (100 μL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80-120 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry