Recombinant Mouse IFNAR1 Protein (Glu27-Thr429), C-10×His-tagged

Product Description

Cat

IMP-10429

Official Symbol

Ifnar1

Product Overview

Recombinant Mouse IFN-alpha/beta R1 protein (Glu27-Thr429) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family and functions as an antiviral factor.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-10×His

Predicted N Terminal

Glu27

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 47.1 kDa
SDS-PAGE: 60-70 kDa, reducing conditions

Protein length

Glu27-Thr429

Bio-activity

Measured by its ability to inhibit Limitin-mediated protection of L-929 mouse fibroblast cells viral lysis. The ED50 for this effect is 0.3-1.2 μg/mL in the presence of 30 pg/mL of Recombinant Mouse Limitin/IFN-zeta.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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