Description
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Mass
Predicted Molecular Mass: 104.5 kDa (single chain), 80.7 kDa (alpha subunit) & 23.2 kDa (beta subunit)
SDS-PAGE: 160-170 kDa, 120-130 kDa & 45-52 kDa, reducing conditions.
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) IGF-I R/IGF1R is present at 0.5 μg/mL, the concentration of rmIGF-I that produces 50% of the optimal binding response is found to be approximately 10-60 ng/mL.
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
