Recombinant Mouse IL-17A (Thr22-Ala158) and IL-17F (Arg21-Ala153) Protein
Product Description
Cat
IMP-10522
Official Symbol
Il17a
Product Overview
Recombinant Mouse IL-17A (Thr22-Ala158) and IL-17F (Arg21-Ala153, with an N-terminal Met) protein without tag was expressed in E. coli.
Description
IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F. Members of this family demonstrate a structural motif termed a cysteine-knot that also characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Thr22 (IL-17A)&Met (IL-17F)
Form
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 14.9 kDa (IL-17A) & 15.5 kDa (IL-17F), reducing conditions
Protein length
IL-17A (Thr22-Ala158) and IL-17F (Arg21-Ala153)
Bio-activity
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.075-0.375 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info