Recombinant Mouse Il13ra1 Protein, C-His-tagged
Product Description
Cat
IMP-4639
Official Symbol
Il13ra1
Product Overview
Recombinant extracellular domain (Met 1-Thr 340) of mouse IL13Rα1 (NP_598751.3) precursor was fused with the a polyhistidine tag at the C-terminus.
Description
Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2 and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
Expression System
HEK293
Species
Mouse
Tag
C-His
Predicted N Terminal
Ala 26
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse IL13Rα1 consists of 326 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IL13Rα1 is approximately 50-55 kDa due to glycosylation.
Protein length
Met1-Thr340
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 98 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized mouse IL13RA1-His at 10μg/mL (100 μL/well) can bind human Fc-IL13, the EC50 of human Fc-IL13 is 0.5-1.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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