Recombinant Mouse IL17A protein, C-His tag

Product Description

Cat

IMP-1161

Official Symbol

IL17A

Product Overview

Recombinant Mouse IL17A(Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A; CTLA8; IL17)(Thr22-Ala158) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Mouse

Tag

C-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 16.2kDa, and migrates as 17-26kDa under reducing conditions.

Protein length

Thr22-Ala158

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1.25 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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