Recombinant Mouse IL17B Protein (His21-Phe180)

Product Description

Cat

IMP-10496

Official Symbol

Il17b

Product Overview

Recombinant Mouse IL-17B protein (His21-Phe180) with an N-terminal Met was expressed in E. coli.

Description

The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. Alternative splicing results in multiple splice variants.

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 18.2 kDa (monomer)

Protein length

His21-Phe180

Bio-activity

Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 0.4-2 μg/mL.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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