Recombinant Mouse IL17F Protein (Arg21-Ala153)

Product Description

Cat

IMP-10526

Official Symbol

Il17f

Product Overview

Recombinant Mouse IL-17F protein (Arg21-Ala153) with an N-terminal Met was expressed in E. coli.

Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 15 kDa (monomer)
SDS-PAGE: 16 kDa, reducing conditions

Protein length

Arg21-Ala153

Bio-activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-120 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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