Recombinant Mouse IL17F Protein (Arg21-Ala153)

Product Description

Cat

IMP-10526

Official Symbol

Il17f

Product Overview

Recombinant Mouse IL-17F protein (Arg21-Ala153) with an N-terminal Met was expressed in E. coli.

Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 15 kDa (monomer)
SDS-PAGE: 16 kDa, reducing conditions

Protein length

Arg21-Ala153

Bio-activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-120 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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