Recombinant Mouse IL17F Protein (Arg29-Ala161)
Product Description
Cat
IMP-10528
Official Symbol
Il17f
Product Overview
Recombinant Mouse IL-17F protein (Arg29-Ala161) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Arg29
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 15 kDa (monomer)
SDS-PAGE: 17-27 kDa, under reducing conditions
Protein length
Arg29-Ala161
Bio-activity
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 40-240 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 40-240 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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