Recombinant Mouse Il1a Protein (115-270aa), C-His-Tagged
Product Description
Cat
IMP-9515
Official Symbol
Il1a
Product Overview
Recombinant mouse IL-1 alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
IL-1 alpha/IL-1F1, also known as interleukin-1 alpha, is a member of the interleukin-1 cytokine family. Cytokines are protein signaling compounds that are the main mediators of the immune response. This protein is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Also, it stimulates thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation.
Expression System
Baculovirus
Species
Mouse
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
19.0 kDa (165 aa)
Protein length
115-270
AA Sequence
<ADP>SAPYTYQSDLRYKLMKLVRQKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/mL (determined by Absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Mouse IL-1 alpha/IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.15 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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