Recombinant Mouse IL1b protein, N-His-GST Tag
Product Description
Cat
IMP-2866
Official Symbol
IL1b
Product Overview
Recombinant Mouse IL1b protein(Val118~Ser269), fused with N-terminal His and GST Tag
, was expressed in E. coli.
Expression System
E. coli
Species
Mouse
Tag
N-His-GST Tag
Form
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose
Molecular Mass
46kDa
Protein length
Val118~Ser269
Purity
> 95%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
IL-8 levels in the cell supernatant of A549 induced by IL-1β

Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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