Recombinant Mouse IL1b protein, N-His Tag

Product Description

Cat

IMP-2864

Official Symbol

IL1b

Product Overview

Recombinant Mouse IL1b protein(P10749)(Val118~Ser269), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Mouse

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

14kDa

Protein length

Ile576~Pro678

Purity

> 97%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

IL-8 levels in the cell supernatant of A549 induced by IL-1β

Interleukin 1 beta (IL-1β) also known as leuk°Cytic pyrogen, leuk°Cytic endogenous mediator, mononuclear cell factor, lymph°Cyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations (0.1ng/mL-1000ng/mL) of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β have shown in Figure1.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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