Recombinant Mouse IL1b protein, N-His Tag

Product Description

Cat

IMP-2864

Official Symbol

IL1b

Product Overview

Recombinant Mouse IL1b protein(P10749)(Val118~Ser269), fused with N-terminal His Tag, was expressed in E. coli.

Expression System

E. coli

Species

Mouse

Tag

N-His Tag

Form

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Molecular Mass

14kDa

Protein length

Ile576~Pro678

Purity

> 97%

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Western Blot 1

SDS-PAGE

Bioactivity-ELISA 1

IL-8 levels in the cell supernatant of A549 induced by IL-1β

Interleukin 1 beta (IL-1β) also known as leuk°Cytic pyrogen, leuk°Cytic endogenous mediator, mononuclear cell factor, lymph°Cyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations (0.1ng/mL-1000ng/mL) of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β have shown in Figure1.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry