Recombinant Mouse IL1R1 Protein (Leu20-Lys338), C-hFc-tagged

Product Description

Cat

IMP-10489

Official Symbol

Il1r1

Product Overview

Recombinant Mouse IL-1 RI protein (Leu20-Lys338) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Leu20

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 63.8 kDa (monomer)
SDS-PAGE: 95-110 kDa, reducing conditions

Protein length

Leu20-Lys338

Bio-activity

Measured by its ability to inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.005-0.015 μg/mL of IL-1 sRI/Fc Chimera will inhibit 50% of the biological response due to 50 pg/mL of rmIL-1a.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry