Recombinant Mouse Il2 (C160S) Protein (21-169aa), Tag Free

Product Description

Cat

IMP-9355

Official Symbol

Il2

Product Overview

Recombinant mouse IL2 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Description

IL2 (Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by Tcells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Form

Liquid in Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Molecular Mass

17.3 kDa (150 aa) confirmed by MALDI-TOF

Protein length

21-169

AA Sequence

MAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFSQSIISTSPQ

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Applications

SDS-PAGE,Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by BCA assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Mouse IL-2 (C160S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.9 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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