Recombinant Mouse IL3RA Protein (Ser17-Lys331), C-hFc-tagged
Product Description
Cat
IMP-10453
Official Symbol
Il3ra
Product Overview
Recombinant Mouse IL-3R alpha/CD123 protein (Ser17-Lys331) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Sf 21 cells.
Description
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
Expression System
Sf 21 cells
Species
Mouse
Tag
C-hFc
Predicted N Terminal
Ser17
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 61.0 kDa (monomer)
SDS-PAGE: 70-80 kDa, reducing conditions
Protein length
Ser17-Lys331
Bio-activity
Measured by its ability to inhibit the IL-3-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.03-0.15 μg/mL in the presence of 2.5 μg/mL Recombinant Mouse IL-3 R beta Fc Chimera and 2 ng/mL of Recombinant Mouse IL-3.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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