Recombinant Mouse IL5RA Protein (Asp18-His339), C-hFc-tagged
Product Description
Cat
IMP-10449
Official Symbol
Il5ra
Product Overview
Recombinant Mouse IL-5 R alpha protein (Asp18-His339) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc
Predicted N Terminal
Asp18
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 63.2 kDa (monomer)
SDS-PAGE: 75-85 kDa, 55 kDa and 35 kDa under reducing conditions
Protein length
Asp18-His339
Bio-activity
Measured by its binding ability in a functional ELISA. When 0.5 μg/mL of Recombinant Mouse IL-5 R alpha/CD125 Fc Chimera is immobilized onto a goat anti-human IgG Fc antibody coated plate, the concentration of biotinylated recombinant mouse IL-5 that produces 50% of the optimal binding response is found to be approximately 4-16 ng/mL
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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