Recombinant Mouse Il5ra Protein, C-His-tagged
Product Description
Cat
IMP-4747
Official Symbol
Il5ra
Product Overview
Recombinant mouse IL5Ra (P21183) (Met1-Val328) was expressed with a C-terminal polyhistidine tag.
Description
Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors.
Expression System
Insect cell
Species
Mouse
Tag
C-His
Predicted N Terminal
Asp 18
Form
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse IL5Ra comprises 321 amino acids and has a predicted molecular mass of 36.8 kDa. The apparent molecular mass of the protein is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein length
Met1-Val328
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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