Recombinant Mouse IL6R protein, N-His Tag
Product Description
Cat
IMP-2389
Official Symbol
IL6R
Product Overview
Recombinant Mouse IL6R protein(P22272)(Ala52~Ala204), fused with N-terminal His Tag, was expressed in E. coli.
Expression System
E. coli
Species
Mouse
Tag
N-His Tag
Form
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Protein length
Tyr504-Lys517
Purity
> 97%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
The binding activity of IL6R with IL6

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the ass°Ciation of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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