Recombinant Mouse Il9 Protein, C-His-tagged
Product Description
Cat
IMP-4768
Official Symbol
Il9
Product Overview
Recombinant mouse IL9 (NP_032399.1) (Met1-Pro144) was expressed with a polyhistidine tag at the C-terminus.
Description
Interleukin 9, also known as IL-9, is a cytokine (cell signaling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects the differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells synergizes with TGF-β1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and the absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight the role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.
Expression System
Insect cell
Species
Mouse
Tag
C-His
Predicted N Terminal
Gln 19
Form
Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0, 10 % glycerol, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse IL9 consists of 137 amino acids and predicts a molecular mass of 15.6 kDa.
Protein length
Met1-Pro144
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is typically 0.5-2 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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