Recombinant Mouse IL9 Protein (Gln19-Pro144)
Product Description
Cat
IMP-10435
Official Symbol
Il9
Product Overview
Recombinant Mouse IL-9 protein (Gln19-Pro144) without tag was expressed in Sf 21 cells.
Description
The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
Expression System
Sf 21 cells
Species
Mouse
Tag
Tag Free
Predicted N Terminal
No results obtained, sequencing might be blocked: Gln19 is predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 14.2 kDa
SDS-PAGE: Multiple bands between 16-25 kDa, reducing conditions
Protein length
Gln19-Pro144
Bio-activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.500-5.00 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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