IMP-9685

Klrk1

Recombinant Mouse NKG2D protein (Phe94-Val232) with a Human IgG1 (Pro100-Lys330) Fc tag at N-terminus was expressed in Mouse myeloma cell line.

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

Mouse myeloma cell line

Mouse

N-hFc

Met

Lyophilized from a 0.2 μm filtered solution in PBS.

Predicted Molecular Mass: 42.7 kDa (monomer)
SDS-PAGE: 55-60 kDa, reducing conditions

Phe94-Val232

Measured by its binding ability in a functional ELISA. Immobilized rmNKG2D/Fc Chimera at 4 μg/mL (100 μL/well) can bind biotinylated rmRae-1 gamma/Fc Chimera with a linear range of 1.56-100 ng/mL.

<0.10 EU/μg of the protein by the LAL method.

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain

Disulfide-linked homodimer

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitute at 100 μg/mL in sterile PBS.