Recombinant Mouse LAG3 Protein (Gly24-Leu442), C-mFc-tagged

Product Description

Cat

IMP-9760

Official Symbol

Lag3

Product Overview

Recombinant Mouse LAG-3 protein (Gly24-Leu442) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Gly24

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 72.4 kDa (monomer)
SDS-PAGE: 85-100 kDa, reducing conditions

Protein length

Gly24-Leu442

Bio-activity

Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS. Reconstitute 30 minutes prior to use with minimal agitation.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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