Recombinant Mouse LDLR Protein (Ala22-Arg790, Ala23Val and Cys27Gly), C-10×His-tagged
Product Description
Cat
IMP-9723
Official Symbol
Ldlr
Product Overview
Recombinant Mouse LDLR protein (Ala22-Arg790, Ala23Val and Cys27Gly) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-10×His
Predicted N Terminal
Ala22
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 85.7 kDa
SDS-PAGE: 130-135 kDa, reducing conditions
Protein length
Ala22-Arg790, Ala23Val and Cys27Gly
Bio-activity
Measured by its binding ability in a functional ELISA. When Human LDL is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse LDLR that produces 50% of the optimal binding response is 0.4-4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human LDL is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse LDLR that produces 50% of the optimal binding response is 0.4-4 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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