Recombinant Mouse Lgals3 Protein (Ala2-Ile264), Tag Free
Product Description
Cat
IMP-9661
Official Symbol
Lgals3
Product Overview
Recombinant Mouse Galectin-3 protein (Ala2-Ile264) without tag was expressed in E. coli.
Description
Predicted to enable several functions, including IgE binding activity; advanced glycation end-product receptor activity; and signaling receptor binding activity. Involved in negative regulation of T cell receptor signaling pathway; negative regulation of endocytosis; and negative regulation of lymphocyte activation. Acts upstream of or within extracellular matrix organization and skeletal system development. Located in several cellular components, including external side of plasma membrane; glial cell projection; and immunological synapse. Is expressed in several structures, including alimentary system; genitourinary system; respiratory system; skeleton; and skin. Used to study fatty liver disease. Human ortholog(s) of this gene implicated in asthma. Orthologous to human LGALS3 (galectin 3).
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Ala2
Form
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 27.3 kDa
Protein length
Ala2-Ile264
Bio-activity
Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is 5-20 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, 2 to 8 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is 5-20 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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