Recombinant Mouse LGALS3 Protein (Met1-Ile264)
Product Description
Cat
IMP-9937
Official Symbol
Lgals3
Product Overview
Recombinant Mouse Galectin-3 protein (Met1-Ile264) without tag was expressed in Human embryonic kidney cell.
Description
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Expression System
HEK293
Species
Mouse
Tag
Tag Free
Predicted N Terminal
No results obtained
Form
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 27-36 kDa, reducing conditions
Protein length
Met1-Ile264
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in water.
Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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