Recombinant Mouse LGALS3 Protein (Met1-Ile264)
Product Description
Cat
IMP-9937
Official Symbol
Lgals3
Product Overview
Recombinant Mouse Galectin-3 protein (Met1-Ile264) without tag was expressed in Human embryonic kidney cell.
Description
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Expression System
HEK293
Species
Mouse
Tag
Tag Free
Predicted N Terminal
No results obtained
Form
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Molecular Mass
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 27-36 kDa, reducing conditions
Protein length
Met1-Ile264
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in water.
Bioactivity-ELISA 1
Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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