Recombinant Mouse LGR4 Protein (Gly20-Thr544), C-mFc-tagged
Product Description
Cat
IMP-9908
Official Symbol
Lgr4
Product Overview
Recombinant Mouse Lgr4/GPR48 protein (Gly20-Thr544) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body.
Expression System
CHO
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gly20&Ala25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 84.6 kDa (monomer)
SDS-PAGE: 100-130 kDa, reducing conditions
Protein length
Gly20-Thr544
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Mouse Lgr4/GPR48 Fc Chimera binds with an apparent Kd <2nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Data Sheet MSDS
Datasheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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