Recombinant Mouse LGR4 Protein (Gly20-Thr544), C-mFc-tagged
Product Description
Cat
IMP-9908
Official Symbol
Lgr4
Product Overview
Recombinant Mouse Lgr4/GPR48 protein (Gly20-Thr544) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body.
Expression System
CHO
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gly20&Ala25
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 84.6 kDa (monomer)
SDS-PAGE: 100-130 kDa, reducing conditions
Protein length
Gly20-Thr544
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Mouse Lgr4/GPR48 Fc Chimera binds with an apparent Kd <2nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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