Recombinant Mouse LIF protein

Product Description

Cat

IMP-1308

Official Symbol

LIF

Product Overview

Recombinant Mouse LIF protein(P09056) was expressed in E. coli.

Expression System

E. coli

Species

Mouse

Tag

N/A

Form

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.

Molecular Mass

Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Protein length

180 aa

AA Sequence

SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

Endotoxin

Less than 1 EU/μg of rMuLIF as determined by LAL method.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.2 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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