Recombinant Mouse LRRC32 Protein (Ile18-Asn628), C-6×His-tagged

Product Description

Cat

IMP-9856

Official Symbol

Lrrc32

Product Overview

Recombinant Mouse LRRC32/GARP protein (Ile18-Asn628) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies.

Expression System

CHO

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Ile18

Form

Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, PEG 3350 and CHAPS.

Molecular Mass

Predicted Molecular Mass: 67.4 kDa
SDS-PAGE: 70-80 kDa, reducing conditions

Protein length

Ile18-Asn628

Bio-activity

Measured by its binding ability in a functional ELISA. When recombinant mouse LRRC32/GARP is immobilized at 5 μg/mL, recombinant human Latent TGF-beta binds with an apparent Kd <25 nM.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 300 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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