Recombinant Mouse LRRC32 Protein (Ile18-Asn628), C-6×His-tagged
Product Description
Cat
IMP-9856
Official Symbol
Lrrc32
Product Overview
Recombinant Mouse LRRC32/GARP protein (Ile18-Asn628) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies.
Expression System
CHO
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Ile18
Form
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, PEG 3350 and CHAPS.
Molecular Mass
Predicted Molecular Mass: 67.4 kDa
SDS-PAGE: 70-80 kDa, reducing conditions
Protein length
Ile18-Asn628
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant mouse LRRC32/GARP is immobilized at 5 μg/mL, recombinant human Latent TGF-beta binds with an apparent Kd <25 nM.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 300 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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