Description
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 × 10^4 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
