Recombinant Mouse Met Protein (Met1-Asn929), C-Fc and 6×His-tagged
Product Description
Cat
IMP-9831
Official Symbol
Met
Product Overview
Recombinant Mouse HGFR/c-MET protein (Met1-Asn929) with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.
Description
Enables hepatocyte growth factor-activated receptor activity. Involved in cell surface receptor signaling pathway; modulation of chemical synaptic transmission; and positive chemotaxis. Acts upstream of or within several processes, including animal organ development; regulation of cellular response to stress; and regulation of cytokine production. Located in membrane. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and limb. Used to study Gilles de la Tourette syndrome; autism spectrum disorder; breast cancer; and gestational diabetes. Human ortholog(s) of this gene implicated in autistic disorder; autosomal recessive nonsyndromic deafness 97; carcinoma (multiple); and multiple myeloma. Orthologous to human MET (MET proto-oncogene, receptor tyrosine kinase).
Expression System
Sf 21 cells
Species
Mouse
Tag
C-Fc and 6×His
Predicted N Terminal
Glu25 (alpha chain) & Ser307 (beta chain)
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 32 kDa ( alpha chain) & 96 kDa ( beta chain) (monomer)
SDS-PAGE: 35-40 kDa & 100-110 kDa, reducing conditions
Protein length
Met1-Asn929
Bio-activity
Measured by its ability to bind rmHGF in a functional ELISA with an estimated KD <0.2 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Notes
Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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