Recombinant Mouse Met Protein (Met1-Asn929), C-Fc and 6×His-tagged
Product Description
Cat
IMP-9831
Official Symbol
Met
Product Overview
Recombinant Mouse HGFR/c-MET protein (Met1-Asn929) with a human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.
Description
Enables hepatocyte growth factor-activated receptor activity. Involved in cell surface receptor signaling pathway; modulation of chemical synaptic transmission; and positive chemotaxis. Acts upstream of or within several processes, including animal organ development; regulation of cellular response to stress; and regulation of cytokine production. Located in membrane. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and limb. Used to study Gilles de la Tourette syndrome; autism spectrum disorder; breast cancer; and gestational diabetes. Human ortholog(s) of this gene implicated in autistic disorder; autosomal recessive nonsyndromic deafness 97; carcinoma (multiple); and multiple myeloma. Orthologous to human MET (MET proto-oncogene, receptor tyrosine kinase).
Expression System
Sf 21 cells
Species
Mouse
Tag
C-Fc and 6×His
Predicted N Terminal
Glu25 (alpha chain) & Ser307 (beta chain)
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 32 kDa ( alpha chain) & 96 kDa ( beta chain) (monomer)
SDS-PAGE: 35-40 kDa & 100-110 kDa, reducing conditions
Protein length
Met1-Asn929
Bio-activity
Measured by its ability to bind rmHGF in a functional ELISA with an estimated KD <0.2 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Notes
Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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