Recombinant Mouse MSLN Protein (Asp298-Ser600), C-6×His-tagged
Product Description
Cat
IMP-9925
Official Symbol
Msln
Product Overview
Recombinant Mouse Mesothelin protein (Asp298-Ser600) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Expression System
CHO
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Asp298
Form
Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Molecular Mass
Predicted Molecular Mass: 35 kDa
SDS-PAGE: 38-55 kDa, reducing conditions
Protein length
Asp298-Ser600
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag (Catalog 8604-MS) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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