Recombinant Mouse MSLN Protein (Gln36-Leu288), C-6×His-tagged
Product Description
Cat
IMP-9602
Official Symbol
Msln
Product Overview
Recombinant Mouse Mesothelin protein (Gln36-Leu288) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Gln36 inferred from enzymatic pyroglutamate treatment revealing Thr37
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 29 kDa
SDS-PAGE: 29-45 kDa, under reducing conditions
Protein length
Gln36-Leu288
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Osteopontin/OPN is immobilizedat 0.5 μg/mL,100 μL/well, Recombinant Mouse Mesothelin N-Terminal (aa 36-288) binds with an ED50 of 2-12 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Monomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Osteopontin/OPN is immobilizedat 0.5 μg/mL,100 μL/well, Recombinant Mouse Mesothelin N-Terminal (aa 36-288) binds with an ED50 of 2-12 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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