Recombinant Mouse MSTN protein, N-His Tag
Product Description
Cat
IMP-3242
Official Symbol
MSTN
Product Overview
Recombinant Mouse MSTN protein(O08689)(Asp268~Ser376), fused with N-terminal His Tag, was expressed in E. coli.
Expression System
E. coli
Species
Mouse
Tag
N-His Tag
Form
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass
19kDa
Protein length
Met1~Ala162
Purity
> 95%
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
Western Blot

SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
The binding activity of MSTN with FSTL3

Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by my°Cytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MSTN and recombinant mouse FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was shown in Figure 1, and this effect was in a dose dependent manner.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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