Recombinant Mouse OSM Protein (Ala24-Arg206), Tag Free

Product Description

Cat

IMP-9822

Official Symbol

OSM

Product Overview

Recombinant Mouse Oncostatin M (OSM) protein (Ala24-Arg206) with an N-terminal Met was expressed in E. coli.

Description

Enables cytokine activity. Acts upstream of or within several processes, including negative regulation of meiotic nuclear division; positive regulation of peptidyl-tyrosine phosphorylation; and tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Is expressed in several structures, including dorsal aorta; future spinal cord; liver; reproductive system; and submandibular gland. Orthologous to human OSM (oncostatin M).

Expression System

E. coli

Species

Mouse

Tag

Tag Free

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 21 kDa

Protein length

Ala24-Arg206

Bio-activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1 ng/mL.

Endotoxin

<0.01 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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