Recombinant Mouse Pdcd1 protein, C-Fc Tag

Product Description

Cat

IMP-215

Official Symbol

Pdcd1

Product Overview

Recombinant Mouse Pdcd1 protein(AA Leu 25-Gln 167)(Accession # NP_032824) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Mouse PD-1, Fc Tag with a linear range of 0.039-0.625 ug/mL.

Bioactivity-BLI 1

Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L1, His Tag with an affinity constant of 1.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L2, His Tag with an affinity constant of 0.13 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Captured Mouse PD-1, Fc Tag on Protein A Chip can bind Mouse PD-L1, His Tag with an affinity constant of 2.16 uM as determined in SPR assay (Biacore T200).

Bioactivity-SPR 2

Captured Mouse PD-L1, His Tag on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag with an affinity constant of 1.37 uM as determined in SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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