Mouse PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Mouse PD-1, Fc Tag with a linear range of 0.039-0.625 ug/mL.
Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L1, His Tag with an affinity constant of 1.5 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L2, His Tag with an affinity constant of 0.13 uM as determined in BLI assay (ForteBio Octet Red96e).
Captured Mouse PD-1, Fc Tag on Protein A Chip can bind Mouse PD-L1, His Tag with an affinity constant of 2.16 uM as determined in SPR assay (Biacore T200).
Captured Mouse PD-L1, His Tag on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag with an affinity constant of 1.37 uM as determined in SPR assay (Biacore T200).