Recombinant Mouse Pdcd1 protein, C-Fc Tag
Product Description
Cat
IMP-215
Official Symbol
Pdcd1
Product Overview
Recombinant Mouse Pdcd1 protein(AA Leu 25-Gln 167)(Accession # NP_032824) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Mouse
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Leu 25-Gln 167
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Mouse PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Mouse PD-1, Fc Tag with a linear range of 0.039-0.625 ug/mL.

Bioactivity-BLI 1

Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L1, His Tag with an affinity constant of 1.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Mouse PD-1, Fc Tag on Protein A Biosensor, can bind Mouse PD-L2, His Tag with an affinity constant of 0.13 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1
Bioactivity-SPR

Captured Mouse PD-1, Fc Tag on Protein A Chip can bind Mouse PD-L1, His Tag with an affinity constant of 2.16 uM as determined in SPR assay (Biacore T200).

Bioactivity-SPR 2
Bioactivity-SPR

Captured Mouse PD-L1, His Tag on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag with an affinity constant of 1.37 uM as determined in SPR assay (Biacore T200).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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