Recombinant Mouse Pdcd1 protein, C-mFc Tag

Product Description

Cat

IMP-214

Official Symbol

Pdcd1

Product Overview

Recombinant Mouse Pdcd1 protein(AA Ser 21-Gln 167)(Accession # NP_032824) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 43.7 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ser 21-Gln 167

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse PD-1, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Mouse PD-L1, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.313 ug/mL.

Bioactivity-ELISA 2

Immobilized Mouse PD-L2, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.625 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-L1, Fc Tag (HPLC-verified) on AHC Biosensor, can bind Mouse PD-1, Mouse IgG2a Fc Tag with an affinity constant of 378 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Mouse PD-1, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Mouse PD-L2, His Tag with an affinity constant of 0.28 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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