Recombinant Mouse PECAM1 Protein (Glu18-Lys590), C-6×His-tagged
Product Description
Cat
IMP-9776
Official Symbol
Pecam1
Product Overview
Recombinant Mouse CD31/PECAM-1 protein (Glu18-Lys590) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Glu18
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 65.5 kDa (monomer)
SDS-PAGE: 91-100 kDa, reducing conditions
Protein length
Glu18-Lys590
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD31/PECAM-1 is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Integrin alpha 8 beta 1 that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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