Recombinant Mouse PECAM1 Protein (Glu18-Lys590), C-6×His-tagged
Product Description
Cat
IMP-9776
Official Symbol
Pecam1
Product Overview
Recombinant Mouse CD31/PECAM-1 protein (Glu18-Lys590) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Glu18
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 65.5 kDa (monomer)
SDS-PAGE: 91-100 kDa, reducing conditions
Protein length
Glu18-Lys590
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD31/PECAM-1 is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Integrin alpha 8 beta 1 that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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