Recombinant Mouse Pvr Protein, C-hFc& AVI-tagged, Biotinylated

Product Description

Cat

IMP-1392

Official Symbol

Pvr

Product Overview

Recombinant human PVR (NP_081790.1) (Met1-Arg345) was expressed with a c-terminal Fc region of human IgG1 tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.

Expression System

HEK293

Species

Mouse

Tag

C-hFc& AVI

Predicted N Terminal

Asp 29

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human PVR consists of 570 amino acids and predicts a molecular mass of 62.9 kDa.

Protein length

Met1-Arg345

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse CD226-Fch at 10 μg/mL (100 μL/well) can bind mPVR-Fc-Avi, the EC50 of mPVR-Fc-Avi is 15-45 ng/mL. 2. Measured by its ability to bind NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) in functional Elisa.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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