Recombinant Mouse REN Protein (Leu22-Arg402), C-10×His-tagged
Product Description
Cat
IMP-9802
Official Symbol
Ren1
Product Overview
Recombinant Mouse Renin protein (Leu22-Arg402) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-10×His
Predicted N Terminal
Leu22
Form
Supplied as a 0.2 μm filtered solution in MES and NaCl.
Molecular Mass
Predicted Molecular Mass: 43 kDa
SDS-PAGE: 45-55 kDa, reducing conditions
Protein length
Leu22-Arg402
Bio-activity
Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL™ 520. Peti-Peterdi, J. et al. (2009) Physiology 24:88. The specific activity is >20 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.


Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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