Recombinant Mouse Rspo1 Protein (Ser21-Gly209), Tag Free
Product Description
Cat
IMP-9766
Official Symbol
Rspo1
Product Overview
Recombinant Mouse R-Spondin 1 protein (Ser21-Gly209) with an N-terminal Met was expressed in E. coli.
Description
Enables heparin binding activity and signaling receptor binding activity. Involved in positive regulation of canonical Wnt signaling pathway. Acts upstream of or within several processes, including male meiotic nuclear division; positive regulation of non-canonical Wnt signaling pathway; and regulation of male germ cell proliferation. Located in extracellular space and nucleus. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; genitourinary system; and skeleton. Orthologous to human RSPO1 (R-spondin 1).
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 21 kDa
Protein length
Ser21-Gly209
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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