Recombinant Mouse RSPO2 Protein (Met1-Gly205)
Product Description
Cat
IMP-9871
Official Symbol
Rspo2
Product Overview
Recombinant Mouse R-Spondin 2 protein (Met1-Gly205) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants.
Expression System
CHO
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Predicted Gln22, not observed that sequencing might be blocked&Ala32
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 21 kDa & 20 kDa
SDS-PAGE: 26-27 kDa, reducing conditions
Protein length
Met1-Gly205
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.4-2.4 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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