Recombinant Mouse RSPO4 Protein (Ala19-Gln228)
Product Description
Cat
IMP-9798
Official Symbol
Rspo4
Product Overview
Recombinant Mouse R-Spondin 4 protein (Ala19-Gln228) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Expression System
CHO
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Ala19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 23.9 kDa
SDS-PAGE: 32 kDa, reducing conditions
Protein length
Ala19-Gln228
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5-20 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info