Recombinant Mouse RSPO4 Protein (Ala19-Gln228)
Product Description
Cat
IMP-9798
Official Symbol
Rspo4
Product Overview
Recombinant Mouse R-Spondin 4 protein (Ala19-Gln228) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Expression System
CHO
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Ala19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 23.9 kDa
SDS-PAGE: 32 kDa, reducing conditions
Protein length
Ala19-Gln228
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5-20 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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