Description
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Bio-activity
Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. When 5 × 10^4 cells/well are added to mouse Syndecan-1 and Recombinant Human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 2.5-10 μg/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
