Recombinant Mouse Sectm1a Protein (Gln28-Thr165), C-mFc-tagged
Product Description
Cat
IMP-9897
Official Symbol
Sectm1a
Product Overview
Recombinant Mouse SECTM1A protein (Gln28-Thr165) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Predicted to enable cytokine activity. Predicted to be involved in immune response. Predicted to be located in extracellular region and plasma membrane. Predicted to be integral component of membrane. Is expressed in cortical plate. Orthologous to human SECTM1 (secreted and transmembrane 1).
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gln28 predicted: No results obtained, sequencing might be blocked
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 42.6 kDa (monomer)
SDS-PAGE: 55-66 kDa, reducing conditions
Protein length
Gln28-Thr165
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD7 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse SECTM1A Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD7 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse SECTM1A Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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